HBV qPCR Kit - Vitrosens Biotechnology
https://vitrosens.com/wp-content/uploads/2023/05/HBV.png

HBV qPCR Kit

The CHAINFOR HBV qPCR Kit is intended for in vitro quantitative diagnosis of HBV nucleic acids from Blood and Plasma. The CHAINFOR HBV qPCR Kit is a rapid RT-PCR test that enables Real-Time PCR. The CHAINFOR HBV qPCR Kits optimized with a Virasens Viral Nucleic Acid Purification Kit and Virasens Viral RNA Purification Kit.

Advantages

– Ready to use
– One tube reaction
– Quantitative Detection
– Exogenous Internal Control
– One-step reaction based experiment procedures
– Specificity is _ 99 %
– Accuracy >%95
– Simple, rapid and accurate detection
– High sensitivity detection

Box Content

– HBV Mix 1
– HBV Mix 2
– HBV Quantification standard 1
– HBV Quantification standard 2
– HBV Quantification standard 3
– HBV Quantification standard 4
– HBV Internal Control
– Negative Control

Details

The CHAINFOR HBV qPCR Kit is intended for in vitro quantitative diagnosis of HBV nucleic acids from blood, and biopsy samples. The CHAINFOR HBV qPCR Kit is a rapid RT-PCR test that enables Real-Time PCR. The kit also has the internal control based on the readout of the HEX channel.

The CHAINFOR HBV qPCR Kit targets P gene in the HBV genome based on readout of the FAM channel. In addition, the kits include highly processive Hot start Taq polymerase enzyme. The CHAINFOR HBV qPCR Kit provides RT-qPCR results in less than 60 minutes.

According to clinical studies, the CHAINFOR HBV qPCR Kit has >95% analytical sensitivity at 20 copies/ml and 97% clinical sensitivity.

The CHAINFOR HBV qPCR Kit was analyzed for cross-reactivity and interference substances, and the results of the analyses showed no cross-reactivity or interference with any of the substances tested.

The CHAINFOR HBV qPCR Kit is compatible with those Real-Time PCR systems: Thermo Scientific Quant Studio 5, BMS Mic, Biorad CFX Connect, Biorad CFX96, Hi-Media.

If you would like to learn more about PCR and PCR kits, you can find more information below.

Polymerase chain reaction (PCR) is a common molecular biology technique used in many disciplines to amplify and detect deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) sequences. In the traditional method, (DNA) cloning and amplification can take days. However, the polymerase chain reaction (PCR) method can reduce this time to hours or even less than an hour. In addition, the polymerase chain reaction (PCR) is compassionate and requires minimal templates for detecting and amplifying specific sequences. Unlike many other tests, the polymerase chain reaction (PCR) can detect many diseases even in their earliest stages.
In contrast to other tests, the polymerase chain reaction (PCR) tests can detect even the smallest amount of pathogens. In addition, the amplification process makes finding pathogens in the sample easier. The HBV qPCR Kit, for example, is designed for disease detection.
The polymerase chain reaction (PCR) test for HBV is a molecular test that looks for the genetic material of HBV, the virus that causes HBV, from the analysis of upper respiratory samples. HBV PCR kits are produced for this process. HBV qPCR Kit contain the materials needed for the test.
There are many types of polymerase chain reaction (PCR), and specialized kits such as Real-Time PCR kits and RTq-PCR kits are also available.
The steps are in three parts: denaturation, annealing, and elongation, as in standard polymerase chain reaction (PCR). However, unlike polymerase chain reaction (PCR), qPCR allows the phases to be observed in real-time. Since the reactions that occur can be monitored in real-time, it is also called Real-Time Polymerase Chain Reaction. Since the reactions that occur can be observed in real-time, it is also called Real-Time Polymerase Chain Reaction. There are two methods for qPCR. These methods are dye-based qPCR and probe-based qPCR. In dye-based qPCR, aka fluorescent labeling, is used to quantify amplified deoxyribonucleic acid (DNA) molecules using a dsDNA-binding dye. Fluorescence is measured during each cycle. As the number of deoxyribonucleic acids (DNA) increases, the fluorescence signal increases. In this way, deoxyribonucleic acid (DNA) can be measured in real-time. In probe-based qPCR, multiple targets can be measured in samples. However, this requires the design and optimization of target-specific probes in addition to primers. The most commonly used probe is a hydrolysis probe, which involves the use of a fluorophore and quencher, but there are many different probe designs.
Thanks to qPCR kits created for different purposes, research can be done for the desired objective.
qPCR kits (Quantitative PCR kits or Real-Time PCR kits) are kits that contain the mixtures required for gene expression studies. qPCR kits include deoxyribonucleic acid (DNA) polymerase, dNTPs, MgCl2, amplifiers, stabilizers, intercalation dye, reference dye, primers, and c-deoxyribonucleic acid (cDNA) template required for qPCR reactions. In addition, one-step or two-step qPCR kits for ribonucleic acid (RNA) templates include a reverse transcriptase (also known as RT-qPCR kits). There are qPCR kits for many different purposes, such as the HBV qPCR Kit.

 

https://vitrosens.com/wp-content/uploads/2023/05/HBV.png

Specification

Intended use Detection of SARS-CoV-2 antigen
Storage 2-30℃ / 35.6-86℉
Specimen type Nasal swab
Time to result 15-30 minutes
Shelf life 24 months
Sensitivity 93.1%
Specificity 100.0%

Video

.embed-container { position: relative; padding-bottom: 56.25%; height: 0; overflow: hidden; max-width: 100%; } .embed-container iframe, .embed-container object, .embed-container embed { position: absolute; top: 0; left: 0; width: 100%; height: 100%; }

Order Information

Ref No.
Target
Storage
Kit Size

CFP02

HBV qPCR Kit

-15°C~-20°C

100 T

footer-logo

Vitrosens Biotechnology is a high-tech company in Turkey founded for the development, manufacture, and delivery of in vitro diagnostic devices (IVD) to the world.
Follow USVITROSENS on Social Media
For our updated news and products follow us on social media.

vitrosens logo new

Vitrosens Biotechnology is a high-tech company in Turkey founded for the development, manufacture, and delivery of in vitro diagnostic devices (IVD) to the world.
vitrosens.com
Human Health
Animal Health
Follow USVITROSENS on Social Media
For our updated news and products follow us on social media.