The CHAINFOR SARS-CoV-2 Detection RT-qPCR Kit is intended for in vitro qualitative diagnosis of SARS-CoV-2 nucleic acids from nasopharyngeal aspirate/lavage, bronchoalveolar lavage, nasopharyngeal swab, sputum, and oropharyngeal swab. The CHAINFOR SARS-CoV-2 Detection RT-qPCR Kit is a rapid RT-PCR test that enables reverse transcription and Real-Time PCR. The CHAINFOR SARS-CoV-2 Detection RT-qPCR Kit is optimized with a viral transport medium (VTFOR) or viral nucleic acid transport medium (VNFOR).
In an area where the COVID-19 virus is not circulating, a positive RT-qPCR result for at least two distinct targets on the COVID-19 viral genome, at least one of which is preferably specific to the COVID-19 virus, is indicated. Where the COVID-19 virus is widespread, an RT-qPCR result for a single distinct target, such as the N gene, is considered sufficient. The CHAINFOR S SARS-CoV-2 Detection RT-qPCR Kit targets N1 and N2 genes in the SARS-CoV-2 genome based on readout of the FAM channel. The kit also targets the RNase P gene in the human genome as an internal control based on the readout of the HEX channel. In addition, the kits include highly processive Reverse Transcriptase and Hot start Taq polymerase enzymes. The CHAINFOR SARS-CoV-2 Detection RT-qPCR Kit provides RT-qPCR results in less than 60 minutes.
According to clinical studies, the CHAINFOR SARS-CoV-2 Detection RT-qPCR Kit has >95% analytical sensitivity at 20 copies/ml and 97% clinical sensitivity.
The CHAINFOR SARS-CoV-2 Detection RT-qPCR Kit was analyzed for cross-reactivity and interference substances, and the results of the analyses showed no cross-reactivity or interference with any of the substances tested.
The CHAINFOR SARS-CoV-2 Detection RT-qPCR Kit is compatible with those Real-Time PCR systems: Thermo Scientific Quant Studio 5, BMS Mic, Biorad CFX Connect, Biorad CFX96, Biorad CFX Touch, Applied Bi-osystem ABI 7500, Applied Biosystem ABI 7500 Fast, Applied Biosystem ABI StepOne, Applied Biosystem ABI StepOne Plus, Roche LightCycler 480, Roche LightCycler 96, Qiagen Rotor Gene Q, Qiagen Rotor Gene Series, Corbett Realtime PCR, Slan Realtime PCR, Tianlog Gentier 96E, Tianlong Gentier 96R, Stratagene Mx3500p, Azure Biosystems™ Cielo™ 3 and 6 real-time PCR Systems.
If you would like to learn more about PCR and PCR kits, you can find more information below.
Polymerase chain reaction (PCR) is a common molecular biology technique used in many disciplines to amplify and detect deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) sequences. In the traditional method, (DNA) cloning and amplification can take days. However, the polymerase chain reaction (PCR) method can reduce this time to hours or even less than an hour. In addition, the polymerase chain reaction (PCR) is compassionate and requires minimal templates for detecting and amplifying specific sequences. Unlike many other tests, the polymerase chain reaction (PCR) can detect many diseases even in their earliest stages.
In contrast to other tests, the polymerase chain reaction (PCR) tests can detect even the smallest amount of pathogens. In addition, the amplification process makes finding pathogens in the sample easier. The SARS-CoV-2 PCR kit, for example, is designed for disease detection.
The polymerase chain reaction (PCR) test for SARS-CoV-2 is a molecular test that looks for the genetic material of SARS-CoV-2, the virus that causes COVID-19, from the analysis of upper respiratory samples. This test uses the polymerase chain reaction (PCR) technology to transcribe small amounts of (RNA) from samples into deoxyribonucleic acid (DNA). SARS-CoV-2 PCR kits are produced for this process. SARS-CoV-2 PCR kits contain the materials needed for the test.
There are many types of polymerase chain reaction (PCR), and specialized kits such as Real-Time PCR kits and RTq-PCR kits are also available.
Reverse Transcription Quantitative Polymerase Chain Reaction (RT-qPCR) is used for many nucleic acid detections, characterization, and quantification applications. First, ribonucleic acid (RNA) transcripts are measured by reverse transcribing them into c-deoxyribonucleic acid (cDNA), then qPCR is performed. The steps are in three parts: denaturation, annealing, and elongation, as in standard polymerase chain reaction (PCR). However, unlike polymerase chain reaction (PCR), qPCR allows the phases to be observed in real-time. Since the reactions that occur can be monitored in real-time, it is also called Real-Time Polymerase Chain Reaction. Since the reactions that occur can be observed in real-time, it is also called Real-Time Polymerase Chain Reaction. There are two methods for qPCR. These methods are dye-based qPCR and probe-based qPCR. In dye-based qPCR, aka fluorescent labeling, is used to quantify amplified deoxyribonucleic acid (DNA) molecules using a dsDNA-binding dye. Fluorescence is measured during each cycle. As the number of deoxyribonucleic acids (DNA) increases, the fluorescence signal increases. In this way, deoxyribonucleic acid (DNA) can be measured in real-time. In probe-based qPCR, multiple targets can be measured in samples. However, this requires the design and optimization of target-specific probes in addition to primers. The most commonly used probe is a hydrolysis probe, which involves the use of a fluorophore and quencher, but there are many different probe designs.
Thanks to qPCR kits created for different purposes, research can be done for the desired objective.
qPCR kits (Quantitative PCR kits or Real-Time PCR kits) are kits that contain the mixtures required for gene expression studies. qPCR kits include deoxyribonucleic acid (DNA) polymerase, dNTPs, MgCl2, amplifiers, stabilizers, intercalation dye, reference dye, primers, and c-deoxyribonucleic acid (cDNA) template required for qPCR reactions. In addition, one-step or two-step qPCR kits for ribonucleic acid (RNA) templates include a reverse transcriptase (also known as RT-qPCR kits). There are qPCR kits for many different purposes, such as the SARS-CoV-2 PCR kit.