Estradiol is the most biologically active form of estrogen hormones. In women, it is secreted by the ovaries and has direct effects on the reproductive system. Measurement of estradiol levels is helpful in the diagnosis of infertility, management of pre- and postmenopausal periods, menstrual irregularities, polycystic ovary syndrome (PCOS) and other hormonal disorders.

Adrenal hyperplasia, early puberty, and some ovarian, adrenal, and testicular tumors can cause high estradiol levels. Both women and men can experience symptoms of high estradiol, but they manifest differently: Irregular menstrual periods, exhaustion, weight gain, fibrous cysts in the breasts or low mood.
When a woman enters menopause, ovulation stops and estradiol levels drop. Low estradiol levels can also be very disruptive in everyday life: Hot flashes, night sweats, mood swings, irregular menstrual periods, insomnia and painful urination.
Estradiol (E2) Rapid Test Kit Principle and Technology
The Estradiol (E2) Rapid Test Kit is an in vitro diagnostic tool that quickly, sensitively, and quantitatively measures estradiol levels in serum or plasma. This kit provides healthcare professionals with a practical and quick method for hormone evaluation.
The Estradiol Rapid Test Kit uses fluorescence immunochromatography technology, which works on the principle of competitive binding. The fluorescently labeled anti-E2 antibodies on the kit react with estradiol in the sample. The amount of estradiol in the sample is inversely proportional to the fluorescence intensity in the test line; The greater the amount of estradiol, the less fluorescence appears on the test line.

Steps of Use of the Kit
Easy-to-use and fast Estradiol (E2) Rapid Test Kit steps:
- Sampling: 30 μL of serum or plasma is taken.
- Mixing: The sample is thoroughly mixed with the diluent provided with the kit (stirring slowly for 60 seconds).
- Sample Loading: 100 μL of the mixture is loaded into the sample area of the test card.
- Incubation and Measurement: After the sample is loaded, the card is placed in the immunofluorescence analyzer and the result is read automatically after 10 minutes.

Estradiol (E2) Rapid Test Kit Advantages
- Quick Result: Test results are ready in just 10 minutes.
- High Precision and Reliability: It provides strong support to clinical diagnosis with its quantitative measurement sensitivity and accuracy.
- Ease of Use: Measurement is done quickly and easily with simple steps, without the need for complex procedures.
- Practical Application: Ideal for routine use in clinical settings and laboratories.
- Optimum Storage Conditions: It has stable and easy storage conditions at room temperature (2-30°C).
The Estradiol Rapid Test Kit provides rapid assessment of the patient’s health status and timely interventions. With this kit, the efficiency and speed of healthcare professionals in diagnosis and treatment processes are increased.
Conclusion
Vitrosens’ Estradiol (E2) Rapid Test Kit (FIA) equips healthcare professionals with swift and accurate estradiol measurements, enhancing diagnostic precision and patient management in reproductive health and hormone-related disorders. By delivering rapid, reliable results and streamlining clinical workflows, Vitrosens is transforming the approach to estradiol assessment worldwide.
Ready to elevate your practice with advanced hormonal testing solutions?
📧 Contact the Vitrosens team today for evaluation kits and expert technical support!
Key References:
- World Health Statistics 2025: Monitoring Health for the SDGs, Sustainable Development Goals
- BENDIS, Peyton Christine, et al. The impact of estradiol on serotonin, glutamate, and dopamine systems. Frontiers in neuroscience, 2024, 18: 1348551.
- MAUVAIS-JARVIS, Franck, et al. Metabolic benefits afforded by estradiol and testosterone in both sexes: clinical considerations. The Journal of Clinical Investigation, 2024, 134.17.
- Vitrosens Biotechnology. Estradiol (E2) Rapid Test Kit (FIA) – Technical Manual.