HPV qPCR Kit

The CHAINFOR HPV qPCR Kit targets P gene in the HPV 16 and 18 genome based on readout of the ROX and Cy5  channels respectively, other 16 HPV variants are detected with FAM channel .  The kit also has the internal control based on the readout of the HEX channel. In addition, the kits include highly processive Hot start Taq polymerase enzyme. The CHAINFOR HPV qPCR Kit provides RT-qPCR results in less than 60 minutes.

According to clinical studies, the CHAINFOR HPV qPCR Kit has >95% analytical sensitivity at 20 copies/ml and 97% clinical sensitivity.

The CHAINFOR HPV qPCR Kit was analyzed for cross-reactivity and interference substances, and the results of the analyses showed no cross-reactivity or interference with any of the substances tested.

The CHAINFOR HPV qPCR Kitis compatible with those Real-Time PCR systems: Thermo Scientific Quant Studio 5, BMS Mic, Biorad CFX Connect, Biorad CFX96, Hi-Media.

If you would like to learn more about PCR and PCR kits, you can find more information below.

Polymerase chain reaction (PCR) is a common molecular biology technique used in many disciplines to amplify and detect deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) sequences. In the traditional method, (DNA) cloning and amplification can take days. However, the polymerase chain reaction (PCR) method can reduce this time to hours or even less than an hour. In addition, the polymerase chain reaction (PCR) is compassionate and requires minimal templates for detecting and amplifying specific sequences. Unlike many other tests, the polymerase chain reaction (PCR) can detect many diseases even in their earliest stages.
In contrast to other tests, the polymerase chain reaction (PCR) tests can detect even the smallest amount of pathogens. In addition, the amplification process makes finding pathogens in the sample easier. The HPV qPCR Kit, for example, is designed for disease detection.
The polymerase chain reaction (PCR) test for HPV is a molecular test that looks for the genetic material of HCV, the virus that causes HPV. HPV PCR kits are produced for this process. HPV qPCR Kit contain the materials needed for the test.
There are many types of polymerase chain reaction (PCR), and specialized kits such as Real-Time PCR kits and RTq-PCR kits are also available.
The steps are in three parts: denaturation, annealing, and elongation, as in standard polymerase chain reaction (PCR). However, unlike polymerase chain reaction (PCR), qPCR allows the phases to be observed in real-time. Since the reactions that occur can be monitored in real-time, it is also called Real-Time Polymerase Chain Reaction. Since the reactions that occur can be observed in real-time, it is also called Real-Time Polymerase Chain Reaction. There are two methods for qPCR. These methods are dye-based qPCR and probe-based qPCR. In dye-based qPCR, aka fluorescent labeling, is used to quantify amplified deoxyribonucleic acid (DNA) molecules using a dsDNA-binding dye. Fluorescence is measured during each cycle. As the number of deoxyribonucleic acids (DNA) increases, the fluorescence signal increases. In this way, deoxyribonucleic acid (DNA) can be measured in real-time. In probe-based qPCR, multiple targets can be measured in samples. However, this requires the design and optimization of target-specific probes in addition to primers. The most commonly used probe is a hydrolysis probe, which involves the use of a fluorophore and quencher, but there are many different probe designs.
Thanks to qPCR kits created for different purposes, research can be done for the desired objective.
qPCR kits (Qualitative PCR kits or Real-Time PCR kits) are kits that contain the mixtures required for gene expression studies. qPCR kits include deoxyribonucleic acid (DNA) polymerase, dNTPs, MgCl2, amplifiers, stabilizers, intercalation dye, reference dye, primers, and c-deoxyribonucleic acid (cDNA) template required for qPCR reactions. In addition, one-step or two-step qPCR kits for ribonucleic acid (RNA) templates include a reverse transcriptase (also known as RT-qPCR kits). There are qPCR kits for many different purposes, such as the HPV qPCR Kit.